| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Capture immunoassay for LT detection produced by enterotoxigenic Escherichia coli in bacterial isolates
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| Autores: |
Menezes,Caroline Anunciação
Gonçalves,Danielle Silva
Amianti,Jackeline
Fernandes,Irene
Taddei,Carla Romano
Koga,Paula Célia Mariko
Trabulsi,Luiz Rachid
Martinez,Marina Baquerizo
Piazza,Roxane Maria Fontes
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| Data: |
2003-11-01
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| Ano: |
2003
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| Palavras-chave: |
Detection
Polyclonal antibody
Monoclonal antibody
Thermo-labile toxin
E. coli
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| Resumo: |
A capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% for sensitivity, 94% for specificity and 92% for efficiency. Thus, the capture immunoassayis a sensitive tool for detection of E. coli, which produces heat-labile enterotoxin, and is suitable for use in clinical laboratories and epidemiological surveys in developing world.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500004
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822003000500004
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.34 suppl.1 2003
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| Direitos: |
info:eu-repo/semantics/openAccess
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